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Part:BBa_I711025:Design

Designed by: Ranjan Khan   Group: iGEM07_Virginia   (2007-07-16)


Acetoacetyl-CoA -> Butyryl-CoA (through crotonyl-CoA)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1349
    Illegal EcoRI site found at 2731
    Illegal PstI site found at 1918
    Illegal PstI site found at 2803
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1349
    Illegal EcoRI site found at 2731
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 830
    Illegal PstI site found at 1918
    Illegal PstI site found at 2803
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1349
    Illegal EcoRI site found at 2731
    Illegal BglII site found at 754
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1349
    Illegal EcoRI site found at 2731
    Illegal PstI site found at 1918
    Illegal PstI site found at 2803
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1349
    Illegal EcoRI site found at 2731
    Illegal PstI site found at 1918
    Illegal PstI site found at 2803
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 451


Design Notes

None.


Source

Clostridium acetobutylicum ATCC 824

References